龙虎山
客服熱線:400-901-9800          客服QQ:4009019800          技術答疑   技術支持   質量反饋   人才招聘   聯系我們
首頁 > 產品中心 > 一抗 > 產品信息
Mouse Anti-Beta tubulin(Loading Control) antibody (bsm-33034M)
訂購熱線:400-901-9800
訂購郵箱:[email protected]
訂購傳真:010-58129612
技術支持:[email protected]
說明書: 50ul  100ul  200ul
50ul/680.00元
100ul/980.00元
200ul/1780.00元
大包裝/詢價

產品編號 bsm-33034M
英文名稱 Beta tubulin(Loading Control)
中文名稱 微管蛋白β tubulin/Tubulin β單克隆抗體(內參抗體)
別    名 Beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; Beta1 tubulin; Class I beta tubulin; M40; MGC117247; MGC16435; OK/SW cl.56; OK/SWcl.56; TBB5_HUMAN; TUBB 1; TUBB 2; TUBB 5; TUBB; TUBB1; TUBB2; TUBB5; tubulin beta 1 chain; Tubulin beta 2 chain; tubulin beta 5 chain; Tubulin beta chain; Tubulin beta class I; tubulin beta polypeptide; Tubulin beta-5 chain.  
產品類型 內參抗體 
研究領域 細胞骨架  細胞外基質  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 7G3
交叉反應 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, Monkey, Insect, Yeast
產品應用 WB=1:1000-5000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:200-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 55kDa
細胞定位 細胞漿 
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Beta tubulin: 
亞    型 IgG1
純化方法 affinity purified by Protein G
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
產品介紹 This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]

Function:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit:
Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO. Interacts with MX1.

Subcellular Location:
Cytoplasmic, cytoskeleton.

Tissue Specificity:
Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Post-translational modifications:
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).

Similarity:
Belongs to the tubulin family.

SWISS:
P07437

Gene ID:
203068

Database links:

Entrez Gene: 203068 Human

Entrez Gene: 22154 Mouse

Entrez Gene: 29214 Rat

Omim: 191130 Human

SwissProt: P07437 Human

SwissProt: P99024 Mouse

SwissProt: P69897 Rat

Unigene: 636480 Human

Unigene: 2458 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

產品圖片
Sample:
U87MG Cell (Human) Lysate at 40 ug
SW480 Cell (Human) Lysate at 40 ug
Primary: Anti- Beta tubulin (bsm-33034M) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
HL60(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (bsm-33034M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD
Sample:
A431(Human) Cell Lysate at 30 ug
SY5Y(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (bsm-33034M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD
Sample:
HL60(Human) Cell Lysate at 30 ug
U937(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
SH-SY5Y(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (bsm-33034M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
A431(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Lovo(Human) Cell Lysate at 30 ug
Primary: Anti- Beta tubulin (bsm-33034M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin) Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
版權所有 2016-2020 www.egfqj.club 北京博奧森生物技術有限公司 京ICP備05066980號 京公網安備110107000727號
通過國際質量管理體系ISO9001:2008 GB/T19001-2008認證    編號:00115Q211807R1M/1100
龙虎山 786753528414757210943220801611490415761450642906668506193533332437578762749194445340699858862452998234 (function(){ var bp = document.createElement('script'); var curProtocol = window.location.protocol.split(':')[0]; if (curProtocol === 'https') { bp.src = 'https://zz.bdstatic.com/linksubmit/push.js'; } else { bp.src = 'http://push.zhanzhang.baidu.com/push.js'; } var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(bp, s); })();