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Rabbit Anti-GAPDH-Loading Control antibody (bs-2188R)
訂購熱線:400-901-9800
訂購郵箱:[email protected]
訂購傳真:010-58129612
技術支持:[email protected]
說明書: 50ul  100ul  200ul
20ul/480.00元
50ul/680.00元
100ul/980.00元
200ul/1780.00元
大包裝/詢價

產品編號 bs-2188R
英文名稱 GAPDH-Loading Control
中文名稱 3-磷酸甘油醛脫氫酶抗體
別    名 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde 3 phosphate dehydrogenase liver; Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.  
Specific References  (11)     |     bs-2188R has been referenced in 11 publications.
[IF=2.14] Song, Yong, et al. "Increased expression of the pluripotency markers sex-determining region Y-box 2 and Nanog homeobox in ovarian endometriosis." Reproductive Biology and Endocrinology 12.1 (2014): 42.  WB ;  Human.  
[IF=0.99] Shao, Guangcan, et al. "Construction of the plasmid coding for the expression of the EGFP?M?IL?2 (88Arg, 125Ala) fusion protein and the anti?tumor effects exerted by the fusion protein in HeLa-60 cells." Oncology Letters.  WB ;  Human.  
[IF=1.48] Hou, Yu Sen, et al. "Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein." Molecular Medicine Reports.  WB ;  Human.  
[IF=2.30] Zhang, Xiangliang, et al. "MicroRNA-216a enhances the radiosensitivity of pancreatic cancer cells by inhibiting beclin-1-mediated autophagy." Oncology Reports.  WB ;  
[IF=2.09] Tan, Shifan, et al. "miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy." International Journal of Molecular Medicine.  WB ;  
[IF=1.55] Jia, Lianqun, et al. "Effects of Tanshinone IIA on the modulation of miR?33a and the SREBP?2/Pcsk9 signaling pathway in hyperlipidemic rats." Molecular Medicine Reports (2016).  WB ;  Rat.  
[IF=2.11] Yan, Lijie, et al. "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." Journal of cardiovascular pharmacology 64.4 (2014): 310-317.  WB ;  Human.  
[IF=1.28] YIN, Zhi-hong, et al. "Expression and tissue distribution analysis of Angiotensin II in sheep (Ovis aries) skins associated with white and black coat colors." Journal of Zhejiang University Science B 1 (2016).  WB ;  Sheep.  
[IF=4.38] Sun, Pengchao, et al. "SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer." International Journal of Nanomedicine 12 (2017): 2657.  WB ;  Human.  
[IF=2.49] Wang, Hong, et al. "Osthole shows the potential to overcome P-glycoprotein?mediated multidrug resistance in human myelogenous leukemia K562/ADM cells by inhibiting the PI3K/Akt signaling pathway." Oncology reports 35.6 (2016): 3659-3668.  WB ;  Human.  
[IF=4.46] Yan, Lijie, et al. "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." Journal of cardiovascular pharmacology 64.4 (2014): 310-317.  WB ;  Human.  
產品類型 內參抗體 
研究領域 免疫學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Rabbit, 
產品應用 WB=1:2000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 37kDa
細胞定位 細胞核 細胞漿 細胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 GAPDH protein of rabbit: 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
產品介紹 Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 38kDa subunits.

Function:
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Subunit:
Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.

Subcellular Location:
Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.

Post-translational modifications:
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated (Probable).
Sulfhydration at Cys-152 increases catalytic activity.

Similarity:
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

SWISS:
P04406

Gene ID:
100009074

Database links:

Entrez Gene: 493876 Cat

Entrez Gene: 374193 Chicken

Entrez Gene: 403755 Dog

Entrez Gene: 2597 Human

Entrez Gene: 100042025 Mouse

Entrez Gene: 14433 Mouse

Entrez Gene: 396823 Pig

Entrez Gene: 100009074 Rabbit

Entrez Gene: 24383 Rat

Entrez Gene: 685186 Rat

Entrez Gene: 380259 Xenopus laevis

Entrez Gene: 448356 Xenopus tropicalis

Entrez Gene: 317743 Zebrafish

Omim: 138400 Human

SwissProt: Cat

SwissProt: P00356 Chicken

SwissProt: Q28259 Dog

SwissProt: P70685 Guinea pig

SwissProt: P04406 Human

SwissProt: P16858 Mouse

SwissProt: P00355 Pig

SwissProt: P46406 Rabbit

SwissProt: P04797 Rat

SwissProt: P51469 Xenopus laevis

SwissProt: Q5XJ10 Zebrafish

Unigene: 544577 Human

Unigene: 592355 Human

Unigene: 598320 Human

Unigene: 304088 Mouse

Unigene: 309092 Mouse

Unigene: 317779 Mouse

Unigene: 343110 Mouse

Unigene: 392463 Mouse

Unigene: 392480 Mouse

Unigene: 414470 Mouse

Unigene: 458138 Mouse

Unigene: 458416 Mouse

Unigene: 475698 Mouse

Unigene: 129558 Rat

Unigene: 91450 Rat

Unigene: 995 Xenopus laevis

Unigene: 35640 Zebrafish



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

甘油醛-3-磷酸脫氫酶(Glyceraldehyde 3 phosphate dehydrogenase,GAPDH)是糖酵解(glycolysis)過程中的關鍵酶。除了在胞質中作為糖酵解的酶以外,有證據表明哺乳動物細胞中的GAPDH參與了多種胞內生化過程,包括膜融合(membrane fusion)、微管成束(microtubule bundling)、磷酸轉移酶(phosphotransferase)激活、核內RNA出核、DNA復制與DNA修復。一些生理因素,諸如低氧(hypoxia)和尿糖(diabetes),可以增加GAPDH在特定細胞中的表達。GAPDH存在于幾乎所有的組織中,以高水平持續表達。 GAPDH(甘油醛-3-磷酸脫氫酶)是參與糖酵解的一種關鍵酶,由4個30-40kDa的亞基組成。
產品圖片
Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (bs-2188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Sample:
Hela Cell (Human) Lysate at 40 ug
MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti- GAPDH (bs-2188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Sample:
A549(Human) Cell Lysate at 30 ug
U2os(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (bs-2188R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Sample:
Heart (Rat) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Primary: Anti- GAPDH (bs-2188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti-GAPDH (bs-2188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-GAPDH (bs-2188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
Sample:
Muscle (Rat) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Primary: Anti- GAPDH (bs-2188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Sample: Muscle (Mouse) Lysate at 40 ug
Primary: Anti- GAPDH (bs-2188R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- GAPDH(bs-2188R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Sample:
U251(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-GAPDH (bs-2188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated(bs-2188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated(bs-2188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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